Description:

PL Taq Mix is a ready-to-use 2x mix developed for fast and highly-specific PCR. The advanced formulation of PL. Taq Mix exhibits more robust amplification than other commonly used polymerases, delivering a very high yield over a wide range of PCR templates and also at significantly faster PCR reaction times without compromising PCR specificity or yield.

These features make PL Taq Mix the ideal choice for most routine assays. PL Taq Mix contains all the necessary reagents required for trouble-free PCR set up. including pl taq butter, dNTPs, MgCI2' enhancers and stabilizers.

PL Taq Mix is supplied in a convenient all-in-one-tube format and only requires the addition of template, primers and water, thereby reducing the number of pipetting steps and facilitating increased efficiency, throughput and reproducibility.

Description

Quantum Hot Start Mastermix is a ready-to-use 2x mix for fast, highly-specific, hot-start PCR.

Quantum Hot Start Mastermix is powerd by antibody mediated hot-start and does not possess polymerase activity during the reaction set-up, thus reducing non-specific amplification.

The advanced formulation of Quantum Hot Start Mastermix allows fast cycling conditions to be used, highly reducing the reaction time without compromising PCR specificity and yield, making it ideal for high-throughput.

Quantum Hot Start Mastermix contains all the reagents including PL Taq buffer, dNTPs, MgCI2, enhancers and stabilizers necessary for trouble-free PCR reaction set up. The products is supplied conveniently all-in-one tube to reduce the number of pipetting steps and to facilitate increased efficiency, throughput and reproducibility. This makes Quantum Hot Start Mastermix perfect for techniques such as Multiplex PCR that require the ability to define a set of common reaction parameters to all of the primer sets in the reaction, with comparable extension efficiencies, Even though the amplicons are different sizes. Quantum Hot Start Mastermix enables success in Multiplex PCR at the first attempt and can even be used for fast multiplex PCR, without special kits, buffer or optimization.

Description

Mercury DNA Polymerase is a heat-activated thermostable DNA polymerase. Mercury DNA. Polymerase provides extremely high yield and improved specificity when compared to standard polymerases and can eliminate the presence of non-specifics, such as primer-dimers and mis-primed products. Mercury DNA Polymerase is inactive at room temperature and therefore prior to PCR cycling, requires activation by heat treatment for 10 minutes. This facilitates flexibility in reaction setup. Including premixing of PCR reagents at room temperature. Subsequently, the reaction can be handled according to the user's existing protocols for thermostable DNA polymerases.

Components

 Mercury DNA Polymerase

 10x Mercury Buffer

 50mM MgCI2 Solution

 Unit Definition

 One unit will incorporate 10nmoles of dNTPs in 30min at 72̊C.

Description

Grand High Fidelity DNA Polymerase is a thermostable enzyme possessing 5'-3' DNA polymerase and 3'-5' proofreading exonuclease activities, offering high fidelity Grand High Fidelity DNA Polymerase provides high fidelity with an error-rate of 3.0 x 10-6, to five blunt-ended amplicons of up to 5Kb in length.

Grand High Fidelity DNA Polymerase is supplied with 10x Reaction Buffer containing MgSO4' which provides optimal final reaction conditions (1.5mM Mg2+ ) for most experiments. In order to allow optimization of reaction conditions, additional MgCI2 is provided. One unit will incorporate 10nmoles of dNTPs in 30min at 72̊C.

Components

 Grand High Fidelity DNA Polymerase

 10x Grand Buffer

 50mM MgCI2 Solution

Description

Silver Flash Reverse Transcriptase is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability, with no loss of activity following 1 week at room temperature. Silver Flash Reverse Transcriptase is highly sensitive even when the amount of template is a limiting factor, with highly efficient and sensitive transcription, from as little as 10pg, up to 2mg of RNA.

Silver Flash Reverse Transcriptase is suitable for first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.

Components

 Silver Flash Reverse Transcriptase

 5x Reaction Buffer

Description

A set of ready-to-use molecular grade dNTP solutions consisting of 4 separate 100mM solutions of dATP, dGTP, dCTP, and dTTP, at pH 7.5 and supplied as lithium salts in purified water.

The dNTP Mix is designed to save hands-on time for researchers and minimize the possibility of contamination and pipetting errors.

Storage Condition

PLdNTP Set can be stored for 24 months at -20̊C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended.